Day six of the challenge saw our riders cycling from Manchester to Sheffield over the infamous Snake Pass, which includes a four mile climb to a height of 1,680ft — one of the toughest in the UK. At the start of the day the riders were joined at the Malmaison in Manchester was Grant McKenzie – GM Mal Manchester, who was taking the baton on to Sheffield. First stop of the day was the other NHS Blood and Transplant blood donor centre in Manchester, which proved difficult to find. Then it was east towards the Pennines, a journey interrupted when one of the bike's chains snapped. The ride up the Snake Pass from Glossop was slow, but everyone made it up and their reward was some superb scenery including the lake that was used for practice by the Dambusters pilots, followed by an eight mile descent into Sheffield. After the usual tea and biscuits welcome at the blood donor centre and some more bike maintenance, the team retired to their hotel.

Distance covered: 43 miles.

If you want to follow Mike and Paul's progress read their blog. If you want to sponsor them go to www.bloodbikes.co.uk